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Potential in vitro and in vivo colon specific anticancer activity on HCT- 116 Xenograft Nude mice model: Targeted delivery using enteric coated folate modify nanoparticles

หน่วยงาน Central Drug Research Institute (CDRI), India

รายละเอียด

ชื่อเรื่อง : Potential in vitro and in vivo colon specific anticancer activity on HCT- 116 Xenograft Nude mice model: Targeted delivery using enteric coated folate modify nanoparticles
นักวิจัย : Khatik, Renuka , Dwivedi, Pankaj , Reddy, V B , Sharma, Komal , Chuttani, Krishna , Mishra, A K , Dwivedi, A K
คำค้น : Nanoparticles , Curcumin , Nude mice , apoptosis and colon cancer
หน่วยงาน : Central Drug Research Institute (CDRI), India
ผู้ร่วมงาน : -
ปีพิมพ์ : 2558
อ้างอิง : RSC Advances, 2015, 5(21), 16507-16520 , http://hdl.handle.net/123456789/1435
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : CSIR-CDRI Communication No. 8892
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Aim: The aim of the study was to develop a drug delivery system for specific targeting in colon cancer. We have developed Eudragit S-100 (ES) coated folic acid (FA) conjugated gliadin (Gd) delivery system for the effective targeting to the over expressed folate receptors (FRs) in colon cancer. Method: The FA conjugate with Gd (FA-Gd) was synthesized and characterized by FTIR and 1H NMR, this developed conjugated was used to prepare curcumin (CU) loaded nanoparticles (NP) by desolvation method. Result: FA-CU-GdNP was further coated with ES and ES-FA-CU-GdNP was obtained. The ES-FA-CU-GdNP were also capable of inducing cell caspase dependent apoptosis in Caco-2 cell lines and exhibit DNA intercalating activity. In therapeutic experiments the ES-FA-CU-GdNP was administered orally to HCT-116 tumor bearing nude mice. In vivo data of bio-distribution showed that ES-FA-CU-GdNP delivered maximum amount of NP in the colon and tumor, respectively after 12 hours, reflecting its targeting potential to the colon and tumor. Gamma scintigraphy study suggested that ES-FA-CU-GdNP at low pH and released NP slowly at pH 7.4 in the colon. Conclusion: These studies provide evidences that ES-FA-CU-GdNP holds promise to address over-expressed FRs in colorectal cancer and were found to be safe for oral administration for prolonged duration.

บรรณานุกรม :
Khatik, Renuka , Dwivedi, Pankaj , Reddy, V B , Sharma, Komal , Chuttani, Krishna , Mishra, A K , Dwivedi, A K . (2558). Potential in vitro and in vivo colon specific anticancer activity on HCT- 116 Xenograft Nude mice model: Targeted delivery using enteric coated folate modify nanoparticles.
    : Central Drug Research Institute (CDRI), India.
Khatik, Renuka , Dwivedi, Pankaj , Reddy, V B , Sharma, Komal , Chuttani, Krishna , Mishra, A K , Dwivedi, A K . 2558. "Potential in vitro and in vivo colon specific anticancer activity on HCT- 116 Xenograft Nude mice model: Targeted delivery using enteric coated folate modify nanoparticles".
    : Central Drug Research Institute (CDRI), India.
Khatik, Renuka , Dwivedi, Pankaj , Reddy, V B , Sharma, Komal , Chuttani, Krishna , Mishra, A K , Dwivedi, A K . "Potential in vitro and in vivo colon specific anticancer activity on HCT- 116 Xenograft Nude mice model: Targeted delivery using enteric coated folate modify nanoparticles."
    : Central Drug Research Institute (CDRI), India, 2558. Print.
Khatik, Renuka , Dwivedi, Pankaj , Reddy, V B , Sharma, Komal , Chuttani, Krishna , Mishra, A K , Dwivedi, A K . Potential in vitro and in vivo colon specific anticancer activity on HCT- 116 Xenograft Nude mice model: Targeted delivery using enteric coated folate modify nanoparticles. : Central Drug Research Institute (CDRI), India; 2558.