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Wolbachia translation initiation factor-1 is copiously expressed by the adult, microfilariae and infective larvae of Brugia malayi and competitively inhibited by tetracycline

หน่วยงาน Central Drug Research Institute (CDRI), India

รายละเอียด

ชื่อเรื่อง : Wolbachia translation initiation factor-1 is copiously expressed by the adult, microfilariae and infective larvae of Brugia malayi and competitively inhibited by tetracycline
นักวิจัย : Nag, J K , Shrivastava, Nidhi , Tiwari, Manish , Gupta, C L , Bajpai, Preeti , Chahar, Dhanvantri , Bhattacharya, S M
คำค้น : Brugia; Wolbachia , Translation; Initiation Factor , Tetracycline; Protein-Protein Docking
หน่วยงาน : Central Drug Research Institute (CDRI), India
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Acta Trop. 2014,138C:51-59 , http://hdl.handle.net/123456789/1382
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : CSIR-CDRI Communication No. 8689
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The intracellular alphaproteobacteria, Wolbachia, is considered to be a future antimacrofilarial drug target as it is obligatory for filarial endurance. Characterizing wolbachial proteins is necessary to understand wolbachial mechanisms and also for discovering new drug entities. Translation initiation factor-1 (Tl IF-1) is an indispensable prokaryotic factor concerned with bacterial viability. This factor is prioritized as one of the most potent antibacterial drug target. To investigate its role in filarial biology, recombinant Wol Tl IF-1 was purified on metal ion column. The factor was found folded in its monomeric native conformation, and contained a buried fluorophore. Molecular modeling revealed that the factor belonged to the Oligomer Binding family, and consisted of the highly conserved S1 domain with 81.6% of the amino acids occupying the allowed regions in Ramachandran plot. In addition, Wol Tl IF-1 exhibited selective binding to the 30S ribosomal subunit, which declined progressively with tetracycline addition. Tetracycline perturbs interaction of Thr18 and Asn32 of the factor with ribosomal protein S4. The factor was immune-localized in adult, microfilariae (Mf) and infective larvae (L3) of B. malayi by immunoblotting. High expression was also observed in Wolbachia within B. malayi Mf, L3 and female adult parasite along the gravid uteri by the confocal microscopy. Therefore, Wol Tl IF-1 appears to be an essential Wolbachia factor whose inhibition leads to extensive cell apoptosis and premature killing of adult worms, validating the antifilarial potential of the factor.

บรรณานุกรม :
Nag, J K , Shrivastava, Nidhi , Tiwari, Manish , Gupta, C L , Bajpai, Preeti , Chahar, Dhanvantri , Bhattacharya, S M . (2557). Wolbachia translation initiation factor-1 is copiously expressed by the adult, microfilariae and infective larvae of Brugia malayi and competitively inhibited by tetracycline.
    : Central Drug Research Institute (CDRI), India.
Nag, J K , Shrivastava, Nidhi , Tiwari, Manish , Gupta, C L , Bajpai, Preeti , Chahar, Dhanvantri , Bhattacharya, S M . 2557. "Wolbachia translation initiation factor-1 is copiously expressed by the adult, microfilariae and infective larvae of Brugia malayi and competitively inhibited by tetracycline".
    : Central Drug Research Institute (CDRI), India.
Nag, J K , Shrivastava, Nidhi , Tiwari, Manish , Gupta, C L , Bajpai, Preeti , Chahar, Dhanvantri , Bhattacharya, S M . "Wolbachia translation initiation factor-1 is copiously expressed by the adult, microfilariae and infective larvae of Brugia malayi and competitively inhibited by tetracycline."
    : Central Drug Research Institute (CDRI), India, 2557. Print.
Nag, J K , Shrivastava, Nidhi , Tiwari, Manish , Gupta, C L , Bajpai, Preeti , Chahar, Dhanvantri , Bhattacharya, S M . Wolbachia translation initiation factor-1 is copiously expressed by the adult, microfilariae and infective larvae of Brugia malayi and competitively inhibited by tetracycline. : Central Drug Research Institute (CDRI), India; 2557.