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Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance.

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance.
นักวิจัย : Zhang, Hongfang , Chong, Huiqing , Ching, Chi Bun , Song, Hao , Jiang, Rongrong
คำค้น : DRNTU::Engineering::Chemical engineering::Biotechnology.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Zhang, H., Chong, H., Ching, C. B., Song, H.,& Jiang, R. (2012). Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance. Applied Microbiology and Biotechnology, 94(4), 1107-1117. , http://hdl.handle.net/10220/12518 , http://dx.doi.org/10.1007/s00253-012-4012-5
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Applied microbiology and biotechnology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

One major challenge in biofuel production, including biobutanol production, is the low tolerance of the microbial host towards increasing biofuel concentration during fermentation. Here, we have demonstrated that Escherichia coli 1-butanol tolerance can be greatly enhanced through random mutagenesis of global transcription factor cyclic AMP receptor protein (CRP). Four mutants (MT1–MT4) with elevated 1-butanol tolerance were isolated from error-prone PCR libraries through an enrichment screening. A DNA shuffling library was then constructed using MT1–MT4 as templates and one mutant (MT5) that exhibited the best tolerance ability among all variants was selected. In the presence of 0.8 % (v/v, 6.5 g/l) 1-butanol, the growth rate of MT5 was found to be 0.28 h−1 while that of wild type was 0.20 h−1. When 1-butanol concentration increased to 1.2 % (9.7 g/l), the growth rate of MT5 (0.18 h−1) became twice that of the wild type (0.09 h−1). Microbial adhesion to hydrocarbon test showed that cell surface of MT5 was less hydrophobic and its cell length became significantly longer in the presence of 1-butanol, as observed by scanning electron microscopy. Quantitative real-time reverse transcription PCR analysis revealed that several CRP regulated, 1-butanol stress response related genes (rpoH, ompF, sodA, manX, male, and marA) demonstrated differential expression in MT5 in the presence or absence of 1-butanol. In conclusion, direct manipulation of the transcript profile through engineering global transcription factor CRP can provide a useful tool in strain engineering.

บรรณานุกรม :
Zhang, Hongfang , Chong, Huiqing , Ching, Chi Bun , Song, Hao , Jiang, Rongrong . (2555). Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance..
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zhang, Hongfang , Chong, Huiqing , Ching, Chi Bun , Song, Hao , Jiang, Rongrong . 2555. "Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance.".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Zhang, Hongfang , Chong, Huiqing , Ching, Chi Bun , Song, Hao , Jiang, Rongrong . "Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance.."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Zhang, Hongfang , Chong, Huiqing , Ching, Chi Bun , Song, Hao , Jiang, Rongrong . Engineering global transcription factor cyclic AMP receptor protein of Escherichia coli for improved 1-butanol tolerance.. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.