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Comparison of alkyl hydroperoxide reductase and two water-forming NADH oxidases from Bacillus cereus ATCC 14579

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Comparison of alkyl hydroperoxide reductase and two water-forming NADH oxidases from Bacillus cereus ATCC 14579
นักวิจัย : Wang, Liang , Chong, Huiqing , Jiang, Rongrong
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Wang, L., Chong, H.,& Jiang, R. (2012). Comparison of alkyl hydroperoxide reductase and two water-forming NADH oxidases from Bacillus cereus ATCC 14579. Applied Microbiology and Biotechnology, 96(5), 1265-1273. , http://hdl.handle.net/10220/12616 , http://dx.doi.org/10.1007/s00253-012-3919-1
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Applied microbiology and biotechnology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Bacillus cereus (B. cereus) is an ubiquitous facultative anaerobic bacterium, and its growth in aerobic environment correlates to the functions of its oxygen defense system. Water-forming NADH oxidase (nox-2) can catalyze the conversion of oxygen to water with concomitant NADH oxidation in anaerobic microorganisms. Here, we report the cloning and characterization of two annotated nox-2 s (nox-2(444) and nox-2(554)) from B. cereus ATCC 14579 and their comparison with another oxidative stress defense system alkyl hydroperoxide reductase (AhpR) from this microbe, which composed of two enzymes—hydrogen peroxide-forming NADH oxidase (nox-1) and peroxidase. Both nox-2 and AhpR catalyze the same reaction in the presence of oxygen. With the stimulation of exogenously added FAD, the maximum activity of nox-1, nox-2(444), and nox-2(554) could reach 27.7 U/mg, 22.9 U/mg, and 2.4 U/mg, respectively, at pH 7.0, 30 °C. Different from nox-1, both nox-2 s were thermotolerant enzymes and could maintain above 87% of their optimum activity at 80 °C, which was not found in other nox-2 s. As for operational stability, all are turnover-limited. Exogenously added reductive reagent dithiothreitol could dramatically increase the total turnover number of nox-2(444) and nox-2(554) by twofold and threefold, respectively, but had no effect on AhpR or nox-1.

บรรณานุกรม :
Wang, Liang , Chong, Huiqing , Jiang, Rongrong . (2555). Comparison of alkyl hydroperoxide reductase and two water-forming NADH oxidases from Bacillus cereus ATCC 14579.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Liang , Chong, Huiqing , Jiang, Rongrong . 2555. "Comparison of alkyl hydroperoxide reductase and two water-forming NADH oxidases from Bacillus cereus ATCC 14579".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Wang, Liang , Chong, Huiqing , Jiang, Rongrong . "Comparison of alkyl hydroperoxide reductase and two water-forming NADH oxidases from Bacillus cereus ATCC 14579."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Wang, Liang , Chong, Huiqing , Jiang, Rongrong . Comparison of alkyl hydroperoxide reductase and two water-forming NADH oxidases from Bacillus cereus ATCC 14579. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.