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Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains
นักวิจัย : Liu, Fang , Soh, Annie Yan Ni , Lim, Yingjie , Mohanram, Harini , Bhattacharjya, Surajit , Xing, Bengang
คำค้น : -
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2555
อ้างอิง : Liu, F., Soh, A. Y. N., Lim, Y., Mohanram, H., Bhattacharjya, S., & Xing, B. (2012). Lipopolysaccharide Neutralizing Peptide–Porphyrin Conjugates for Effective Photoinactivation and Intracellular Imaging of Gram-Negative Bacteria Strains. Bioconjugate Chemistry, 23(8), 1639-1647. , 1043-1802 , http://hdl.handle.net/10220/10770 , http://dx.doi.org/10.1021/bc300203d
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Bioconjugate chemistry
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

A simple and specific strategy based on the bioconjugation of a photosensitizer protophophyrin IX (PpIX) with a lipopolysaccharide (LPS) binding antimicrobial peptide YI13WF (YVLWKRKRKFCFI-Amide) has been developed for the effective fluorescent imaging and photodynamic inactivation of Gram-negative bacterial strains. The intracellular fluorescent imaging and photodynamic antimicrobial chemotherapy (PACT) studies supported our hypothesis that the PpIX-YI13WF conjugates could serve as efficient probes to image the bacterial strains and meanwhile indicated the potent activities against Gram-negative bacterial pathogens especially for those with antibiotics resistance when exposed to the white light irradiation. Compared to the monomeric PpIX-YI13WF conjugate, the dimeric conjugate indicated the stronger fluorescent imaging signals and higher photoinactivation toward the Gram-negative bacterial pathogens throughout the whole concentration range. In addition, the photodynamic bacterial inactivation also demonstrated more potent activity than the minimum inhibitory concentration (MIC) values of dimeric PpIX-YI13WF conjugate itself observed for E. coli DH5a (4 times), S. enterica (8 times), and other Gram-negative strains including antibiotic-resistant E. coli BL21 (8 times) and K. pneumoniae (16 times). Moreover, both fluorescent imaging and photoinactivation measurements also demonstrated that the dimeric PpIX-YI13WF conjugate could selectively recognize bacterial strains over mammalian cells and generate less photo damage to mammalian cells. We believed that the enhanced fluorescence and bacterial inactivation were probably attributed to the higher binding affinity between dimeric photosensitizer peptide conjugate and LPS components on the surface of bacterial strains, which were the results of efficient multivalent interactions.

บรรณานุกรม :
Liu, Fang , Soh, Annie Yan Ni , Lim, Yingjie , Mohanram, Harini , Bhattacharjya, Surajit , Xing, Bengang . (2555). Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Liu, Fang , Soh, Annie Yan Ni , Lim, Yingjie , Mohanram, Harini , Bhattacharjya, Surajit , Xing, Bengang . 2555. "Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Liu, Fang , Soh, Annie Yan Ni , Lim, Yingjie , Mohanram, Harini , Bhattacharjya, Surajit , Xing, Bengang . "Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2555. Print.
Liu, Fang , Soh, Annie Yan Ni , Lim, Yingjie , Mohanram, Harini , Bhattacharjya, Surajit , Xing, Bengang . Lipopolysaccharide neutralizing peptide–porphyrin conjugates for effective photoinactivation and intracellular imaging of gram-negative bacteria strains. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2555.