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Visualizing the perturbation of cellular cyclic di-GMP levels in bacterial cells

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Visualizing the perturbation of cellular cyclic di-GMP levels in bacterial cells
นักวิจัย : Ho, Chun Loong , Chong, Kavin Shi Jie , Oppong, Jamila Akosua , Chuah, Mary Lay-Cheng , Tan, Suet Mien , Liang, Zhao-Xun
คำค้น : DRNTU::Science::Biological sciences.
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2556
อ้างอิง : Ho, C. L., Chong, K. S. J., Oppong, J. A., Chuah, M. L. C., Tan, S. M., & Liang, Z. X. (2013). Visualizing the Perturbation of Cellular Cyclic di-GMP Levels in Bacterial Cells. Journal of the American Chemical Society, 135(2), 566-569. , http://hdl.handle.net/10220/9326 , http://dx.doi.org/10.1021/ja310497x
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Journal of the American chemical society
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Cyclic di-GMP (c-di-GMP) has emerged as a prominent intracellular messenger that coordinates biofilm formation and pathogenicity in many bacterial species. Developing genetically encoded biosensors for c-di-GMP will help us understand how bacterial cells respond to environmental changes via the modulation of cellular c-di-GMP levels. Here we report the design of two genetically encoded c-di-GMP fluorescent biosensors with complementary dynamic ranges. By using the biosensors, we found that several compounds known to promote biofilm dispersal trigger a decline in c-di-GMP levels in Escherichia coli cells. In contrast, cellular c-di-GMP levels were elevated when the bacterial cells were treated with subinhibitory concentrations of biofilm-promoting antibiotics. The biosensors also revealed that E. coli cells engulfed by macrophages exhibit lower c-di-GMP levels, most likely as a response to the enormous pressures of survival during phagocytosis.

บรรณานุกรม :
Ho, Chun Loong , Chong, Kavin Shi Jie , Oppong, Jamila Akosua , Chuah, Mary Lay-Cheng , Tan, Suet Mien , Liang, Zhao-Xun . (2556). Visualizing the perturbation of cellular cyclic di-GMP levels in bacterial cells.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ho, Chun Loong , Chong, Kavin Shi Jie , Oppong, Jamila Akosua , Chuah, Mary Lay-Cheng , Tan, Suet Mien , Liang, Zhao-Xun . 2556. "Visualizing the perturbation of cellular cyclic di-GMP levels in bacterial cells".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Ho, Chun Loong , Chong, Kavin Shi Jie , Oppong, Jamila Akosua , Chuah, Mary Lay-Cheng , Tan, Suet Mien , Liang, Zhao-Xun . "Visualizing the perturbation of cellular cyclic di-GMP levels in bacterial cells."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2556. Print.
Ho, Chun Loong , Chong, Kavin Shi Jie , Oppong, Jamila Akosua , Chuah, Mary Lay-Cheng , Tan, Suet Mien , Liang, Zhao-Xun . Visualizing the perturbation of cellular cyclic di-GMP levels in bacterial cells. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2556.