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Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis
นักวิจัย : Soo, Jianchow , Zhang, Jing , He, Qiyuan , Agarwal, Shuchi , Li, Hai , Zhang, Hua , Chen, Peng
คำค้น : DRNTU::Science::Biological sciences
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2553
อ้างอิง : Soo, J. C., Zhang, J., He, Q., Agarwal, S., Li, H., Zhang, H., et al. (2010). Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis. Integrative Biology, 2, 250–257. , http://hdl.handle.net/10220/8406 , http://dx.doi.org/10.1039/c0ib00006j , 151699
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Integrative biology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The subunit B of cholera toxin (CTB), which specifically binds with ganglioside GM1 enriched in membrane lipid rafts, is known to interfere with multiple cell functions. However, the specific, stable and spatially defined membrane signaling induced by CTB binding is often difficult to investigate by applying CTB molecules in bulk solution due to quick internalization, elicited intracellular reactions, and homogeneous interaction with the entire cell membrane. Here, we interfaced the neuroendocrine PC12 cells with surface immobilized and patterned CTB molecules, and interrogated the effects of CTB binding on vesicular exocytosis using integrative single-cell study methods. It was discovered that CTB binding facilitates vesicle trafficking, docking and exocytosis in a cholesterol dependent manner. And these effects are probably attributable to the increased membrane GM1 and cholesterol, and enhanced Ca2+ signaling.

บรรณานุกรม :
Soo, Jianchow , Zhang, Jing , He, Qiyuan , Agarwal, Shuchi , Li, Hai , Zhang, Hua , Chen, Peng . (2553). Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Soo, Jianchow , Zhang, Jing , He, Qiyuan , Agarwal, Shuchi , Li, Hai , Zhang, Hua , Chen, Peng . 2553. "Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Soo, Jianchow , Zhang, Jing , He, Qiyuan , Agarwal, Shuchi , Li, Hai , Zhang, Hua , Chen, Peng . "Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2553. Print.
Soo, Jianchow , Zhang, Jing , He, Qiyuan , Agarwal, Shuchi , Li, Hai , Zhang, Hua , Chen, Peng . Surface immobilized cholera toxin B subunit (CTB) facilitates vesicle docking, trafficking and exocytosis. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2553.