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Analysis of the correlation between mesenchymal-like stem cells and invasiveness of MDA-MB-231 cells.

หน่วยงาน Universiti Sains Malaysia, Malaysia

รายละเอียด

ชื่อเรื่อง : Analysis of the correlation between mesenchymal-like stem cells and invasiveness of MDA-MB-231 cells.
นักวิจัย : Khoo, Boon Yin
คำค้น : R735-854 Medical education. Medical schools. Research
หน่วยงาน : Universiti Sains Malaysia, Malaysia
ผู้ร่วมงาน : -
ปีพิมพ์ : -
อ้างอิง : Khoo, Boon Yin Analysis of the correlation between mesenchymal-like stem cells and invasiveness of MDA-MB-231 cells. Project Report. Universiti Sains Malaysia. (Submitted)
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : http://eprints.usm.my/31708/
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

This project that hypothesised the invasiveness of MDA-MB-231 is caused by the presence of CD10S+ cells (mesenchymal-like stem cells) in the cancer cell population, managed to isolate the CD105+ from the cell population of MDA-MB-231 using the MASC Magnetic Cell· Sorting Kit. The project found that removal of the CD10S+ cells from the cell population of MDA-MB-231 did not reduce the growth rate or caused any apoptotic effect to the cell population. Measurement of the invasion rate of the isolated cells (CD10S+) using Transwell Cell Migration Assay was impossible as the isolated cells were easily turned into the original one within a few days. As such, the invasion rate was only measured in the CD10S- cells. The gene expression at mRNA level was also determined in the CD1 OS- cells using real-time PCR. Our project demonstrated that withdrawn of the CD105+ cells from the MDA-MB-231 cell population reduced the rate of the cell invasion, which was accompanying with the reduced expression level of FGF4 mRNA in the cells. The level of FGF4 protein was also found reducing in the conditioned medium of the CD105- cells by ELISA post removal of the CD105+ cells from the cancer cell population.

บรรณานุกรม :
Khoo, Boon Yin . (). Analysis of the correlation between mesenchymal-like stem cells and invasiveness of MDA-MB-231 cells..
    กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia.
Khoo, Boon Yin . . "Analysis of the correlation between mesenchymal-like stem cells and invasiveness of MDA-MB-231 cells.".
    กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia.
Khoo, Boon Yin . "Analysis of the correlation between mesenchymal-like stem cells and invasiveness of MDA-MB-231 cells.."
    กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia, . Print.
Khoo, Boon Yin . Analysis of the correlation between mesenchymal-like stem cells and invasiveness of MDA-MB-231 cells.. กรุงเทพมหานคร : Universiti Sains Malaysia, Malaysia; .