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Quantitative Determination of Microbicidal Spermicide ‘Nonoxynol-9’ in Rabbit Plasma and Vaginal Fluid using LC-ESI-MS/MS: Application to Pharmacokinetic study

หน่วยงาน Central Drug Research Institute (CDRI), India

รายละเอียด

ชื่อเรื่อง : Quantitative Determination of Microbicidal Spermicide ‘Nonoxynol-9’ in Rabbit Plasma and Vaginal Fluid using LC-ESI-MS/MS: Application to Pharmacokinetic study
นักวิจัย : Chhonker, Y S , Chandasana, Hardik , Bala, Veenu , Kumar, Lokesh , Sharma, V L , Gupta, Gopal , Bhatta, R S
คำค้น : Nonoxynol-9 , Spermicide , Microbicide , Pharmacokinetics , LC–MS/MS
หน่วยงาน : Central Drug Research Institute (CDRI), India
ผู้ร่วมงาน : -
ปีพิมพ์ : 2557
อ้างอิง : Journal of Chromatography B, 2014, 965, 127–132 , http://hdl.handle.net/123456789/1416
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : CSIR-CDRI Communication No. 8727
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Nonoxynol-9 (N-9), a microbicidal spermicide, has been in use as an over-the-counter contraceptive since the 1960s. A detailed account of its pharmacokinetic profile using highly sensitive detection method has not been reported yet. We developed and validated a rapid, selective and sensitive high-performance liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) method for N-9 detection in plasma and simulated vaginal fluid. The analytes were quantified using reverse phase Thermo Accucore C18 (150 × 4.6 mm, 5 μm) column with isocratic elution using acetonitrile: 0.1% formic acid in triple distilled water (90:10, v/v) as mobile phase. The ionization was optimized using ESI (+) and selectivity was achieved by tandem mass spectrometric analysis using MRM transition, m/z 617.4→133.2 for N-9 and m/z 180.1→138.1 for phenacetin. The method was linear over the range of 0.195–100 ng/mL. The method was accurate and precise with intra‐batch and inter‐batch accuracy (% bias) of < ± 15% and precision (% CV) of < 15% for N-9. The mean peak plasma concentration (Cmax) 4.87 ± 0.37 ng/mL was achieved 1.0 hr after vaginal application with terminal half-life 1.45 ± 0.07 hr in rabbits. The validated method was successfully applied for pharmacokinetic study of N-9 in rabbits after vaginal administration

บรรณานุกรม :
Chhonker, Y S , Chandasana, Hardik , Bala, Veenu , Kumar, Lokesh , Sharma, V L , Gupta, Gopal , Bhatta, R S . (2557). Quantitative Determination of Microbicidal Spermicide ‘Nonoxynol-9’ in Rabbit Plasma and Vaginal Fluid using LC-ESI-MS/MS: Application to Pharmacokinetic study.
    : Central Drug Research Institute (CDRI), India.
Chhonker, Y S , Chandasana, Hardik , Bala, Veenu , Kumar, Lokesh , Sharma, V L , Gupta, Gopal , Bhatta, R S . 2557. "Quantitative Determination of Microbicidal Spermicide ‘Nonoxynol-9’ in Rabbit Plasma and Vaginal Fluid using LC-ESI-MS/MS: Application to Pharmacokinetic study".
    : Central Drug Research Institute (CDRI), India.
Chhonker, Y S , Chandasana, Hardik , Bala, Veenu , Kumar, Lokesh , Sharma, V L , Gupta, Gopal , Bhatta, R S . "Quantitative Determination of Microbicidal Spermicide ‘Nonoxynol-9’ in Rabbit Plasma and Vaginal Fluid using LC-ESI-MS/MS: Application to Pharmacokinetic study."
    : Central Drug Research Institute (CDRI), India, 2557. Print.
Chhonker, Y S , Chandasana, Hardik , Bala, Veenu , Kumar, Lokesh , Sharma, V L , Gupta, Gopal , Bhatta, R S . Quantitative Determination of Microbicidal Spermicide ‘Nonoxynol-9’ in Rabbit Plasma and Vaginal Fluid using LC-ESI-MS/MS: Application to Pharmacokinetic study. : Central Drug Research Institute (CDRI), India; 2557.