ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs

หน่วยงาน Nanyang Technological University, Singapore

รายละเอียด

ชื่อเรื่อง : STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs
นักวิจัย : Garcia-Alvarez, Gisela , Lu, Bo , Yap, Kenrick An Fu , Wong, Loo Chin , Thevathasan, Jervis Vermal , Lim, Lynette , Ji, Fang , Tan, Kia Wee , Mancuso, James J. , Tang, Willcyn , Poon, Shou Yu , Augustine, George J. , Fivaz, Marc
คำค้น : DRNTU::Science::Biological sciences::Molecular biology
หน่วยงาน : Nanyang Technological University, Singapore
ผู้ร่วมงาน : -
ปีพิมพ์ : 2558
อ้างอิง : Garcia-Alvarez, G., Lu, B., Yap, K. A. F., Wong, L. C., Thevathasan, J. V., Lim, L., et al. (2015). STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs. Molecular biology of the cell, 26(6), 1141-1159. , 1059-1524 , http://hdl.handle.net/10220/25307 , http://dx.doi.org/10.1091/mbc.E14-07-1222
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : Molecular biology of the cell
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

STIMs (STIM1 and STIM2 in mammals) are transmembrane proteins that reside in the endoplasmic reticulum (ER) and regulate store-operated Ca(2+) entry (SOCE). The function of STIMs in the brain is only beginning to be explored, and the relevance of SOCE in nerve cells is being debated. Here we identify STIM2 as a central organizer of excitatory synapses. STIM2, but not its paralogue STIM1, influences the formation of dendritic spines and shapes basal synaptic transmission in excitatory neurons. We further demonstrate that STIM2 is essential for cAMP/PKA-dependent phosphorylation of the AMPA receptor (AMPAR) subunit GluA1. cAMP triggers rapid migration of STIM2 to ER-plasma membrane (PM) contact sites, enhances recruitment of GluA1 to these ER-PM junctions, and promotes localization of STIM2 in dendritic spines. Both biochemical and imaging data suggest that STIM2 regulates GluA1 phosphorylation by coupling PKA to the AMPAR in a SOCE-independent manner. Consistent with a central role of STIM2 in regulating AMPAR phosphorylation, STIM2 promotes cAMP-dependent surface delivery of GluA1 through combined effects on exocytosis and endocytosis. Collectively our results point to a unique mechanism of synaptic plasticity driven by dynamic assembly of a STIM2 signaling complex at ER-PM contact sites.

บรรณานุกรม :
Garcia-Alvarez, Gisela , Lu, Bo , Yap, Kenrick An Fu , Wong, Loo Chin , Thevathasan, Jervis Vermal , Lim, Lynette , Ji, Fang , Tan, Kia Wee , Mancuso, James J. , Tang, Willcyn , Poon, Shou Yu , Augustine, George J. , Fivaz, Marc . (2558). STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs.
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Garcia-Alvarez, Gisela , Lu, Bo , Yap, Kenrick An Fu , Wong, Loo Chin , Thevathasan, Jervis Vermal , Lim, Lynette , Ji, Fang , Tan, Kia Wee , Mancuso, James J. , Tang, Willcyn , Poon, Shou Yu , Augustine, George J. , Fivaz, Marc . 2558. "STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs".
    กรุงเทพมหานคร : Nanyang Technological University, Singapore.
Garcia-Alvarez, Gisela , Lu, Bo , Yap, Kenrick An Fu , Wong, Loo Chin , Thevathasan, Jervis Vermal , Lim, Lynette , Ji, Fang , Tan, Kia Wee , Mancuso, James J. , Tang, Willcyn , Poon, Shou Yu , Augustine, George J. , Fivaz, Marc . "STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs."
    กรุงเทพมหานคร : Nanyang Technological University, Singapore, 2558. Print.
Garcia-Alvarez, Gisela , Lu, Bo , Yap, Kenrick An Fu , Wong, Loo Chin , Thevathasan, Jervis Vermal , Lim, Lynette , Ji, Fang , Tan, Kia Wee , Mancuso, James J. , Tang, Willcyn , Poon, Shou Yu , Augustine, George J. , Fivaz, Marc . STIM2 regulates PKA-dependent phosphorylation and trafficking of AMPARs. กรุงเทพมหานคร : Nanyang Technological University, Singapore; 2558.