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Local anesthetic effect of pellitorine, an active compound isolated from Cha-plu fruit (Piper sarmentosum Roxb.)

หน่วยงาน จุฬาลงกรณ์มหาวิทยาลัย

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ชื่อเรื่อง : Local anesthetic effect of pellitorine, an active compound isolated from Cha-plu fruit (Piper sarmentosum Roxb.)
นักวิจัย : Piyaluk Sapprasert
คำค้น : Local anesthetics , pellitorine , Piperaceae
หน่วยงาน : จุฬาลงกรณ์มหาวิทยาลัย
ผู้ร่วมงาน : Boonyong Tantisira , Mayuree Tantisira , Chulalongkorn University. Graduate School
ปีพิมพ์ : 2546
อ้างอิง : 9741740298 , http://cuir.car.chula.ac.th/handle/123456789/7003
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Thesis (M.Sc.)--Chulalongkorn University, 2003

To investigate local anesthetic effect of a semi-purified compound, containing mainly pellitorine, isolated from piper sarmentosum Roxb. on isolated frog sciatic nerves. In addition, in vivo effect of the test compound was further investigated in mouse tail flick test and twitch-response test in guinea pig's skin. The test compound was dissolved in 0.1 M hydroxypropyl-beta-cyclodextrin in Ringer's solution and being applied onto frog sciatic nerve. It was found that pellitorine in the concentration of 10-25 mM exerted a concentration-dependent manner manner and reversible blockade of supramaximally electrical-evoked action potential of isolated frog sciatic nerves. Complete block electrical-evoked action potential exerted by 25 mM of pellitorine and 30 mM of lidocaine was achieved at 73.75+-2.26 min and 7.50+-0.94 min respectively. Further investigation in mouse tail flick test, using analgesia meter, revealed in vivo local anesthetic effect of test substances which was injected into mouse's tail base. The EC[subscript 50] of pellitorine and lidocaine in this model were found to be 16 and 12 mM, respectively. This observation was subsequently confirmed by twitch response in which the test substances were infiltrated intradermally on the back of guinea pig and pricking was used to probe for response to pain. Apparently, pellitorine (3-14 mM) as well as lidocaine (4-16 mM), elicited analgesic effect with the duration of sensory block of about 30-50 min. Based on the results obtained it can be concluded that pellitorine possesses in vitro as well as in vivo local anesthetic effects. The finding that the degrees of conduction block elicited by pellitorine or lidocaine on frog sciatic nerve were markedly increased in sodium deficient Ringer's solution, suggesting the similar mode of action of theses two compounds. Further investigation on the effect of pellitorine on sodium channel is required to elucidate the mechanism of local anesthetic observed. In addition toxicity which may arise should also be evaluated and that may lead to a discovery of a local anesthetic from natural source.

บรรณานุกรม :
Piyaluk Sapprasert . (2546). Local anesthetic effect of pellitorine, an active compound isolated from Cha-plu fruit (Piper sarmentosum Roxb.).
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Piyaluk Sapprasert . 2546. "Local anesthetic effect of pellitorine, an active compound isolated from Cha-plu fruit (Piper sarmentosum Roxb.)".
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Piyaluk Sapprasert . "Local anesthetic effect of pellitorine, an active compound isolated from Cha-plu fruit (Piper sarmentosum Roxb.)."
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2546. Print.
Piyaluk Sapprasert . Local anesthetic effect of pellitorine, an active compound isolated from Cha-plu fruit (Piper sarmentosum Roxb.). กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2546.