ชื่อเรื่อง | : | Toxin a-producing Clostridium difficile isolated from feces of premature infant, full-term infant, children with anticancer drug and adult with diarrhea |
นักวิจัย | : | Sriwanna Huttayananont |
คำค้น | : | Clostridium difficile , Diarrhea , Toxin A |
หน่วยงาน | : | จุฬาลงกรณ์มหาวิทยาลัย |
ผู้ร่วมงาน | : | Pintip Pongpech , Siripan Wongwanich , Chulalongkorn University. Graduate School |
ปีพิมพ์ | : | 2543 |
อ้างอิง | : | 9743463577 , http://cuir.car.chula.ac.th/handle/123456789/6015 |
ที่มา | : | - |
ความเชี่ยวชาญ | : | - |
ความสัมพันธ์ | : | - |
ขอบเขตของเนื้อหา | : | - |
บทคัดย่อ/คำอธิบาย | : | Thesis (M.Sc.)--Chulalongkorn University, 2000 The prevalence of C.difficile in 4 groups of 443 patients (446 stool specimens) at Siriraj Hospital during the 7 month (October 1998-April 1999) of the study were 15.38% of premature infants, 10.59% of full-term infants, 21.05% of children with anticancer drugs and 25% of the adult patients with diarrhea. It was shown by polymerase chain reaction (PCR) that only 2 isolates from the premature infants and 18 isolates from the adult patients with diarrhea had toxin A gene while enzyme immunoassay (EIA) could not detect txin A production in both 2 isolates from the premature infants and 5 out of 18 isolates from the adult patients. In order to perform the genotyping the chromosomal DNA from all 20 enterotsigenic C. difficile isolates were digested with Sma I restriction endonuclease and separated by pulsed field gel electrophoresis (PFGE). Four different pulsotypes ; A, B, C and D were obtained Fourteen isolates from 11 adult patients with diarrhea were pulsotype a which was the major pulsotype. Seven isolates from 5 adult patients with diarrhea and from 2 premature infants which were only toxin A gene positive by PCR but not toxin A positive by EIA were all pulsotype B or its subtype. PCR has been shown to be a sensitive and useful tool in the detection of the toxigenic C.difficile. PFGE result indicated that the pulsotype A strain was the predominant type and could be related to nosocomial diarrhea. The results obtained should be benefical for patients in both prevention and treatment of this infection. |
บรรณานุกรม | : |
Sriwanna Huttayananont . (2543). Toxin a-producing Clostridium difficile isolated from feces of premature infant, full-term infant, children with anticancer drug and adult with diarrhea.
กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย. Sriwanna Huttayananont . 2543. "Toxin a-producing Clostridium difficile isolated from feces of premature infant, full-term infant, children with anticancer drug and adult with diarrhea".
กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย. Sriwanna Huttayananont . "Toxin a-producing Clostridium difficile isolated from feces of premature infant, full-term infant, children with anticancer drug and adult with diarrhea."
กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2543. Print. Sriwanna Huttayananont . Toxin a-producing Clostridium difficile isolated from feces of premature infant, full-term infant, children with anticancer drug and adult with diarrhea. กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2543.
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