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Physiological responses of black tiger shrimp Penaeus monodon to nitrate in sea water

หน่วยงาน จุฬาลงกรณ์มหาวิทยาลัย

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ชื่อเรื่อง : Physiological responses of black tiger shrimp Penaeus monodon to nitrate in sea water
นักวิจัย : Sarayut Onsanit
คำค้น : Penaeus monodon , Nitrates
หน่วยงาน : จุฬาลงกรณ์มหาวิทยาลัย
ผู้ร่วมงาน : Piamsak Menasveta , Sorawit Powtongsook , Chulalongkorn University. Faculty of Science
ปีพิมพ์ : 2546
อ้างอิง : 9741751184 , http://cuir.car.chula.ac.th/handle/123456789/2901
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ความสัมพันธ์ : -
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Thesis (M.Sc.)--Chulalongkorn University, 2003

This study investigated the physiological responses of black tiger shrimp (Penaeus monodon) to various nitrate concentration in seawater. In the first section, the acute toxicity of nitrate was studied using 17.37+-2.7 g shrimps exposed to nitrate concentrations ranged from 2 to 4449 mgNO3-N/L in 30 psu salinity. It was found that median lethal concentration (LC50) at 96-hr was 2636 mgNO[subscript 3]-N/L. Haemolymph samples were collected from survived shrimps for the determination of total haemocyte counts (THCs), protein, glucose (stress response), phenoloxidase activity (immune response), ammonium, nitrite, and nitrate. The results showed that high THCs was found in shrimp exposed to nitrate concentration below the LC[subscript 50] (2636 mg-N/L) with significant difference (P<0.01 by ANOVA) compared to each nitrate concentration. On the other hand, phenoloxidase activity, haemolymph protein, and haemolymph glucose showed high fluctuation. Analysisof nitrogenous compounds in haemolymph showed that haemolymph ammonium was constant within the range between 12-22 mg-N/L while haemolymph nitrite and nitrate had significant correlation (P<0.05 by ANOVA) with nitrate concentrations in seawater even at nitrate concentration more than 2000 mg-N/L. In the second section, 50 shrimps with initial weight of 24 g were cultured with two closed recirculating seawater systems, each consisted of 7 m[superscript 2] round pond (3m in diameter) and nitrification biofilter tank. The concentration of nitrate in the treatment pond was controlled by nitrate treatment system (the tubular denitrification reactor) attached to nitrification tank while control pond was without nitrate treatment. The results showed that, after 216 days rearing, concentration of nitrate in control pond was accumulated to 86 mg-N/L while nitrate in treatment pond was less than 25 mg-N/L. Survival rate of shrimp in control and treatment ponds were 38% and 54%, respectively. Physiological responses indicated by total haemocyte, haemolymph protein, glucose and phenoloxidase activity were fluctuate with high standard deviation. Highly significant correlation between haemolymph nitrate and nitrate concentration in the water was found, in which the concentration of nitrate in haemolymph was almost equal to the concentration of nitrate in the seawater

บรรณานุกรม :
Sarayut Onsanit . (2546). Physiological responses of black tiger shrimp Penaeus monodon to nitrate in sea water.
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Sarayut Onsanit . 2546. "Physiological responses of black tiger shrimp Penaeus monodon to nitrate in sea water".
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Sarayut Onsanit . "Physiological responses of black tiger shrimp Penaeus monodon to nitrate in sea water."
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2546. Print.
Sarayut Onsanit . Physiological responses of black tiger shrimp Penaeus monodon to nitrate in sea water. กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2546.