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Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids

หน่วยงาน National Institute Of Oceanography (NIO), India

รายละเอียด

ชื่อเรื่อง : Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids
นักวิจัย : Augustine, M.S. , Anas, A. , Das, A.V. , Sreekanth, S. , Jayalekshmi, S.
คำค้น : spectroscopic techniques , toxicity , Amino acids , nanocrystal
หน่วยงาน : National Institute Of Oceanography (NIO), India
ผู้ร่วมงาน : -
ปีพิมพ์ : 2558
อ้างอิง : Spectrochimica Acta Part A:Molecular and Biomolecular Spectroscopy, vol.136; 327–333 , http://drs.nio.org/drs/handle/2264/4687
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Highly luminescent, manganese doped, zinc sulphide (ZnS:Mn) nanocrystals biofunctionalized with chitosan and various aminoacids such as l-citrulline, l-lysine, l-arginine, l-serine, l-histidine and glycine were synthesized by chemical capping co-precipitation method at room temperature, which is a simple and cost effective technique. The synthesized nanocrystals were structurally characterized by TEM, XRD, EDXS and FT-IR spectroscopy techniques. They possess high colloidal stability with strong orange red photoluminescence emission at 598 nm. The intensity of orange red emission has been observed to be maximum in l-citrulline capped ZnS:Mn nanocrystals in which the emission at 420 nm is effectively quenched by surface passivation due to capping. Taking into consideration the prospects of these highly luminescent, bio-compatible ZnS:Mn nanocrystals in bio-imaging applications, cytotoxicity studies were conducted to identify the capping combination which would accomplish minimum toxic effects. ZnS:Mn nanocrystals biofunctionalized with chitosan, l-citrulline, glycine, l-artginine, l-serine and l-histidine showed least toxicity up to 10 nM concentrations in mouse fibroblast L929 cells, which further confirms their cytocompatibility. Also the ZnS:Mn nanocrystals biofunctionalized with l-arginine showed maximum uptake in in vitro studies carried out in human embryonic kidney cells, HEK-293T, which shows the significant role of this particular amino acid in fetoplacental nutrition. The present study highlights the suitability of aminoacid conjugated ZnS:Mn nanocrystals, as promising candidates for biomedical applications

บรรณานุกรม :
Augustine, M.S. , Anas, A. , Das, A.V. , Sreekanth, S. , Jayalekshmi, S. . (2558). Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids.
    : National Institute Of Oceanography (NIO), India.
Augustine, M.S. , Anas, A. , Das, A.V. , Sreekanth, S. , Jayalekshmi, S. . 2558. "Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids".
    : National Institute Of Oceanography (NIO), India.
Augustine, M.S. , Anas, A. , Das, A.V. , Sreekanth, S. , Jayalekshmi, S. . "Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids."
    : National Institute Of Oceanography (NIO), India, 2558. Print.
Augustine, M.S. , Anas, A. , Das, A.V. , Sreekanth, S. , Jayalekshmi, S. . Cytotoxicity and cellular uptake of ZnS:Mn nanocrystals biofunctionalized with chitosan and aminoacids. : National Institute Of Oceanography (NIO), India; 2558.