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Cloning and expression of polyketide synthase genes from cassia alata, plumbago indica and rheum tataricum

หน่วยงาน จุฬาลงกรณ์มหาวิทยาลัย

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ชื่อเรื่อง : Cloning and expression of polyketide synthase genes from cassia alata, plumbago indica and rheum tataricum
นักวิจัย : Supachai Samappito
คำค้น : Cloning , Plant molecular biology , Gene expression
หน่วยงาน : จุฬาลงกรณ์มหาวิทยาลัย
ผู้ร่วมงาน : Wanchai De-Eknamkul , Chulalongkorn University. Faculty of Pharmaceutical Sciences
ปีพิมพ์ : 2545
อ้างอิง : 9741713401 , http://cuir.car.chula.ac.th/handle/123456789/2040
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Thesis (Ph.D.)--Chulalongkorn University, 2002

Studies on cloning and expression of polyketide synthases (PKSs) have been performed using Thai medicinal plants producing anthraquinones (Cassia alata and Rheum tataricum) and naphthoquinones (Plumbago indica). Three cDNAs encoding very similar but unique isoforms of chalcone synthase [EC 2.3.1.74] were isolated from a cDNA library prepared from RNA from root tissue of C. alata. Gene transcript for these three type III polyketide synthases was found to accumulate predominantly in root. The heterologously expressed enzymes accepted a broad starter-coenzyme A (CoA) and together with the co-substrate malonyl-CoA in vitro assays, formed multiple products. C. alata accumulates the flavonoids quercetin, naringenin and kaempferol in roots, suggesting that the in planta function of these enzymes is the biosynthesis of root flavonoids. For P. indica, one cDNA cloned encoding a pyrone synthase, PinPS, was isolated from the root part that correlated with the expression pattern of the PinPS transcripts were found predominantly in roots. Recombinant PinPS was expressed in E. coli and assayed with a various starter-CoA as primers of polyketide synthesis and was found to accepted broad substrate specificity in in vitro assay and produce pyrone product. For R. tataricum, a cDNA encoding a stilbene synthase, RtSTS, was isolated from the rhizomes. Recombinant RtSTS expressed in E. coli and assayed with a various substrate-CoA as primers, could synthesize resveratrol and a trace amount of naringenin chalcone from p-coumaroyl-CoA. These suggested that the enzyme is a resveratrol-type stilbene synthase (EC 2.3.1.95). Moreover, bis-noryangonin and p-coumaroyl triacetic acid lactone (CTAL)-type pyrones were observed in minor amounts in the reaction with p-coumaroyl-CoA. . Using Northern blot analysis, RtSTS transcript was found to be highly expressed in R. tataricum rhizomes.This expression pattern correlated with the occurrence of resveratrol, which was detected in higher amounts in R. tataricum rhizomes compared with leaves and petioles using HPLC

บรรณานุกรม :
Supachai Samappito . (2545). Cloning and expression of polyketide synthase genes from cassia alata, plumbago indica and rheum tataricum.
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Supachai Samappito . 2545. "Cloning and expression of polyketide synthase genes from cassia alata, plumbago indica and rheum tataricum".
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Supachai Samappito . "Cloning and expression of polyketide synthase genes from cassia alata, plumbago indica and rheum tataricum."
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2545. Print.
Supachai Samappito . Cloning and expression of polyketide synthase genes from cassia alata, plumbago indica and rheum tataricum. กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2545.