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Effects of combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) on angiogenesis and wound healing in diabetic mice

หน่วยงาน จุฬาลงกรณ์มหาวิทยาลัย

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ชื่อเรื่อง : Effects of combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) on angiogenesis and wound healing in diabetic mice
นักวิจัย : Supakanda Sukpat
คำค้น : Stem cells , Neovascularization , Wound healing , Diabetes , สเต็มเซลล์ , การเกิดหลอดเลือดใหม่ , การสมานแผล , เบาหวาน
หน่วยงาน : จุฬาลงกรณ์มหาวิทยาลัย
ผู้ร่วมงาน : Suthiluk Patumraj , Nipan Israsena , Chulalongkorn University. Graduate School
ปีพิมพ์ : 2554
อ้างอิง : http://cuir.car.chula.ac.th/handle/123456789/22465
ที่มา : -
ความเชี่ยวชาญ : -
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Thesis (M.Sc.)--Chulalongkorn University, 2011

The effects of combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) on angiogenesis and wound healing in diabetic mice were studied by using Balb/c nude mice. The animals were divided into five groups. Group 1 is control group with implanted fibrin gel (Control; (n=6)). Group 2 is diabetic group with implanted fibrin gel (DM; (n=6)). Group 3 is diabetic group with implanted fibrin gel and 1X10[superscript 6] cells MSCs (DM+MSCs; (n=6)). Group 4 is diabetic wounded group with implanted fibrin gel and 1X10[superscript 6] cells EPCs (DM+EPCs; (n=6)). Group 5 is diabetic wounded group with implanted fibrin gel and combined 0.5X10[superscript 6] cells MSCs and 0.5X10[superscript 6] cells EPCs (DM+MSCs+EPCs; (n=6)). Diabetic groups were induced by injection of streptozotocin ((STZ) 45 mg/kg BW ip. daily for 5 days). After 6-7 weeks, all mice were anesthetized and created bilateral full-thickness excisional skin wounds on the dorso-rostral back (0.6x0.6 sq.cm). Each mouse received fibrin gel or stem cells injection into wound bed. On days 7 and 14 post- wound, the percentage of wound closure (%WC) was accessed by using digital image analysis(Image Pro-Plus). The percentage of capillary density (%CV) was determined by using intravital fluorescence microscopy. The re-epithelialization and the number of neutrophil infiltration were analyzed from wound tissue samples using H&E staining. Tissue vascular endothelial growth factor (VEGF) level was detected by ELISA. The results showed that: 1) Blood sugar level in all diabetic group was increased significantly as compared to Control on day 7 and 14 post-wound. However, body weights of all groups were not significant difference as compared to DM. 2). On day 7 and 14, %WC of all groups increased significantly as compared to DM. 3) On day 7, the %CV of all group increased significantly as compared to DM. The %CV of DM+EPCs+MSCs group was significantly higher than DM+MSCs and DM+EPCs groups (P<0.000). On day 14, the %CV of DM+EPCs+MSCs group was increased significantly as compared to Control. 4) %Re-epithelialization in all groups was not significant difference as compared to DM. 5) On day 7, number of neutrophil infiltration in DM was increased significantly as compared to Control. DM+MSCs, DM+EPCs and DM+EPCs+MSCswere no significant difference when compared to DM group. On day 14, the number of neutrophil infiltration of all groups increased significantly as compared to DM. 6) On day 7, VEGF levels of all groups increased significantly as compared to DM. In conclusion, the present study has demonstrated that the combined EPCs and MSCs can increase VEGF level and increased angiogenesis which lead to reduce number of neutrophil infiltration and enhanced wound healing in diabetic mice model.

บรรณานุกรม :
Supakanda Sukpat . (2554). Effects of combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) on angiogenesis and wound healing in diabetic mice.
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Supakanda Sukpat . 2554. "Effects of combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) on angiogenesis and wound healing in diabetic mice".
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Supakanda Sukpat . "Effects of combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) on angiogenesis and wound healing in diabetic mice."
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2554. Print.
Supakanda Sukpat . Effects of combined endothelial progenitor cells (EPCs) and mesenchymal stem cells (MSCs) on angiogenesis and wound healing in diabetic mice. กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2554.