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Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates

หน่วยงาน National Institute Of Oceanography (NIO), India

รายละเอียด

ชื่อเรื่อง : Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates
นักวิจัย : Ravindran, C. , Naveenan, T. , Varatharajan, G.R.
คำค้น : agro wastes , alkaline cellulase , marine fungi
หน่วยงาน : National Institute Of Oceanography (NIO), India
ผู้ร่วมงาน : -
ปีพิมพ์ : 2553
อ้างอิง : Botanica Marina, vol.53(3); 275-282 , http://drs.nio.org/drs/handle/2264/3637
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Alkaline cellulases were screened from 54 fungal isolates obtained from mangrove leaves and mangrove wood litters as endophytes and wood litter fungi, respectively. Only three fungal isolates tested positive for alkaline cellulases under plate assay, among which Chaetomium sp. (NIOCC 36) was found to grow in a wide range of pH (between 4 and 12). This alkaline tolerant fungus was further tested for production of alkaline cellulases (Beta-endoglucanase, Beta-exoglucanase, Beta-glucosidase) using agricultural and industrial wastes as substrates in submerged fermentation (SMF) and solid state fermentation (SSF) processes. Cotton seed under SSF conditions had maximum enzyme production at high alkaline pH. Cellulase enzymes produced under alkaline cotton seed SSF conditions at 50 degrees C had higher stability and activity than under non-alkaline conditions (range tested: pH 5, 7, 12). The agricultural and industrial wastes used as carbon sources were economical for commercial production of cellulose. The high specific activity, and stability of cellulases under neutral to alkaline pH and high temperature will be of use in various industrial and biotechnological applications.

บรรณานุกรม :
Ravindran, C. , Naveenan, T. , Varatharajan, G.R. . (2553). Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates.
    : National Institute Of Oceanography (NIO), India.
Ravindran, C. , Naveenan, T. , Varatharajan, G.R. . 2553. "Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates".
    : National Institute Of Oceanography (NIO), India.
Ravindran, C. , Naveenan, T. , Varatharajan, G.R. . "Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates."
    : National Institute Of Oceanography (NIO), India, 2553. Print.
Ravindran, C. , Naveenan, T. , Varatharajan, G.R. . Optimization of alkaline cellulase production by the marine-derived fungus Chaetomium sp. using agricultural and industrial wastes as substrates. : National Institute Of Oceanography (NIO), India; 2553.