ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Synthesis of vitamin E glycoside by apha-glucosidase from saccharomyces cerevisiae and cyclodextrin glycosyltransferase from Paenibacillus sp. RB01

หน่วยงาน จุฬาลงกรณ์มหาวิทยาลัย

รายละเอียด

ชื่อเรื่อง : Synthesis of vitamin E glycoside by apha-glucosidase from saccharomyces cerevisiae and cyclodextrin glycosyltransferase from Paenibacillus sp. RB01
นักวิจัย : Pisanu Thanadolsathien
คำค้น : Vitamin E , Glycosides , Cyclodextrins
หน่วยงาน : จุฬาลงกรณ์มหาวิทยาลัย
ผู้ร่วมงาน : Manchumas Prousoontorn , Worawan Bhanthumnavin , Chulalongkorn University. Faculty of Science
ปีพิมพ์ : 2550
อ้างอิง : http://cuir.car.chula.ac.th/handle/123456789/15425
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Thesis (M.Sc.)--Chulalongkorn University, 2007

A novel vitamin E glycoside was synthesized by a two-step enzymatic transglycosylation system, a combination of [alpha]-glucosidase from Saccharomyces cerevisiae and cyclodextrin glycosyltransferase (CGTase) from Paenibacillus sp. RB01. 2-([alpha]-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol (TMG) was synthesized from 2-hydroxyl-2,5,7,8-tetramethylchroman-6-ol (TM) and maltose by [alpha]-glucosidase. TMG was then used as an acceptor to synthesize a novel vitamin E derivative by CGTase using [beta]-cyclodextrin ([beta]-CD) as a donor. A novel product was identified as [alpha]-D-glucopyranosyl-(14)-2-([alpha]-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol (TMG[subscript 2]) by mass spectrometry and nuclear magnetic resonance spectroscopy analysis. When 50 mM potassium phosphate buffer (pH 6.0) containing 0.5% (w/v) of TMG, 1.5% (w/v) of [beta]-CD and 100 U of CGTase was incubated at 50 [degree Celcius] for 4 hours, the percent yield of TMG[subscript 2] reached 47.2% based on the amount of TMG supplied. The solubility in water of TMG[subscript 2] was about 14 times higher than that of TMG and was more than 1 x 10[superscript 4] and 7 x 10[superscript 4] times higher than those of TM and Trolox (6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), respectively. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) free-radical scavenging activity of TMG[subscript 2] was found to be nearly the same as those of TMG, TM and Trolox.

บรรณานุกรม :
Pisanu Thanadolsathien . (2550). Synthesis of vitamin E glycoside by apha-glucosidase from saccharomyces cerevisiae and cyclodextrin glycosyltransferase from Paenibacillus sp. RB01.
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Pisanu Thanadolsathien . 2550. "Synthesis of vitamin E glycoside by apha-glucosidase from saccharomyces cerevisiae and cyclodextrin glycosyltransferase from Paenibacillus sp. RB01".
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Pisanu Thanadolsathien . "Synthesis of vitamin E glycoside by apha-glucosidase from saccharomyces cerevisiae and cyclodextrin glycosyltransferase from Paenibacillus sp. RB01."
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2550. Print.
Pisanu Thanadolsathien . Synthesis of vitamin E glycoside by apha-glucosidase from saccharomyces cerevisiae and cyclodextrin glycosyltransferase from Paenibacillus sp. RB01. กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2550.