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The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus).

หน่วยงาน Southeast Asian Fisheries Development Center, Philippines

รายละเอียด

ชื่อเรื่อง : The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus).
นักวิจัย : Tan, Josefa D. , Adachi, Shinji , Nagahama, Yoshitaka
คำค้น : Carassius auratus , Hormones , Nucleotides , Vitellogenesis , Ovaries , Biosynthesis , Steroids
หน่วยงาน : Southeast Asian Fisheries Development Center, Philippines
ผู้ร่วมงาน : -
ปีพิมพ์ : 2529
อ้างอิง : Tan, J.D., Adachi, S., & Nagahama, Y. (1986). The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus). General and Comparative Endocrinology, 63(1):110-116. , 0016-6480 , http://hdl.handle.net/10862/1200 , 10.1016/0016-6480(86)90188-7
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : General and Comparative Endocrinology
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

The effects of human chorionic gonadotropin (HCG), forskolin, cyclic nucleotides, the phosphodiesterase inhibitors IBMX and theophylline, cyanoketone, and cycloheximide on the production of estradiol-17β by isolated ovarian follicles of vitellogenic goldfish (Carassius auratus) were examined using 18-hr incubations. HCG and all test agents which are known to increase intracellular concentrations of cAMP significantly stimulated the production of estradiol-17β. However, dibutyryl cGMP was unable to stimulate estradiol-17β production at any concentration used (1–10 mM). Cyanoketone at a concentration of 1 μg/ml completely blocked forskolin-induced estradiol-17β production. Even in the presence of cyanoketone, however, forskolin stimulated conversion of exogenous testosterone to estradiol-17β in a dose-dependent manner, suggesting the involvement of an adenylate-cyclase system in the induction of aromatase activation by vitellogenic follicles of goldfish. Cycloheximide also completely abolished HCG-induced estradiol-17β production when this inhibitor was added within the first 1 hr after the addition of HCG. These results provide evidence that the stimulation of estradiol-17β by goldfish vitellogenic follicles in response to HCG is dependent upon the synthesis of new protein.

บรรณานุกรม :
Tan, Josefa D. , Adachi, Shinji , Nagahama, Yoshitaka . (2529). The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus)..
    : Southeast Asian Fisheries Development Center, Philippines.
Tan, Josefa D. , Adachi, Shinji , Nagahama, Yoshitaka . 2529. "The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus).".
    : Southeast Asian Fisheries Development Center, Philippines.
Tan, Josefa D. , Adachi, Shinji , Nagahama, Yoshitaka . "The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus).."
    : Southeast Asian Fisheries Development Center, Philippines, 2529. Print.
Tan, Josefa D. , Adachi, Shinji , Nagahama, Yoshitaka . The in vitro effects of cyclic nucleotides, cyanoketone, and cycloheximide on the production of estradiol-17β by vitellogenic ovarian follicles of goldfish (Carassius auratus).. : Southeast Asian Fisheries Development Center, Philippines; 2529.