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Isolation and cDNA cloning of somatolactin in rabbitfish (Siganus guttatus).

หน่วยงาน Southeast Asian Fisheries Development Center, Philippines

รายละเอียด

ชื่อเรื่อง : Isolation and cDNA cloning of somatolactin in rabbitfish (Siganus guttatus).
นักวิจัย : Ayson, Felix G. , De Jesus, Evelyn T. , Amemiya, Y , Moriyama, S. , Hirano, T. , Kawauchi, H.
คำค้น : Siganus guttatus , Somatolactin , Amino acid sequence , Biochemical analysis , DNA , Evolution , Phylogeny , Pituitary gland
หน่วยงาน : Southeast Asian Fisheries Development Center, Philippines
ผู้ร่วมงาน : -
ปีพิมพ์ : 2542
อ้างอิง : Ayson, F. G., de Jesus, E. G. T., Amemiya, Y., Moriyama, S., Hirano, T., & Kawauchi, H. (1999). Isolation and cDNA cloning of somatolactin in rabbitfish (Siganus guttatus). General and Comparative Endocrinology, 115(2), 292–300. , 0016-6480 , http://hdl.handle.net/10862/996 , 10.1006/gcen.1999.7315
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : General and Comparative Endocrinology , http://www.researchgate.net/publication/12882779_Isolation_and_cDNA_cloning_of_somatolactin_in_rabbitfish_(Siganus_guttatus)/file/50463518b11897a238.pdf
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

We report the isolation and cDNA cloning of somatolactin (SL) from rabbitfish, Siganus guttatus. Rabbitfish SL was isolated from an alkaline extract of the pituitary glands by gel filtration chromatography on Sephadex G-100 and reversed-phase high-performance liquid chromatography. SL was monitored by immunoblotting with flounder SL antiserum. The preparation (yield: 0.86 mg/g wet tissues) contained two immunoreactive bands of 24 and 28 kDa on SDS-PAGE. Overlapping partial cDNA clones corresponding to teleost SLs were amplified by PCR from single-strand cDNA from pituitary glands. Excluding the poly(A) tail, rabbitfish SL cDNA is 1605 bp long. It contains a 693-bp open reading frame encoding a signal peptide of 24 amino acids (aa) and a mature protein of 207 aa. Rabbitfish SL has two possible N-glycosylation sites at positions 11 and 121 and seven half Cys residues. The deduced amino acid sequence shows over 80% identity with those of advanced teleosts like sea bream, red drum, and flounder, 76% with the salmonids, 57% with the eel, and 46% with the goldfish SL.

บรรณานุกรม :
Ayson, Felix G. , De Jesus, Evelyn T. , Amemiya, Y , Moriyama, S. , Hirano, T. , Kawauchi, H. . (2542). Isolation and cDNA cloning of somatolactin in rabbitfish (Siganus guttatus)..
    : Southeast Asian Fisheries Development Center, Philippines.
Ayson, Felix G. , De Jesus, Evelyn T. , Amemiya, Y , Moriyama, S. , Hirano, T. , Kawauchi, H. . 2542. "Isolation and cDNA cloning of somatolactin in rabbitfish (Siganus guttatus).".
    : Southeast Asian Fisheries Development Center, Philippines.
Ayson, Felix G. , De Jesus, Evelyn T. , Amemiya, Y , Moriyama, S. , Hirano, T. , Kawauchi, H. . "Isolation and cDNA cloning of somatolactin in rabbitfish (Siganus guttatus).."
    : Southeast Asian Fisheries Development Center, Philippines, 2542. Print.
Ayson, Felix G. , De Jesus, Evelyn T. , Amemiya, Y , Moriyama, S. , Hirano, T. , Kawauchi, H. . Isolation and cDNA cloning of somatolactin in rabbitfish (Siganus guttatus).. : Southeast Asian Fisheries Development Center, Philippines; 2542.