ridm@nrct.go.th   ระบบคลังข้อมูลงานวิจัยไทย   รายการโปรดที่คุณเลือกไว้

Development of asiatic acid solid lipid nanoparticles for nasal delivery

หน่วยงาน จุฬาลงกรณ์มหาวิทยาลัย

รายละเอียด

ชื่อเรื่อง : Development of asiatic acid solid lipid nanoparticles for nasal delivery
นักวิจัย : Pilaiporn Khunathum
คำค้น : Drug delivery systems , Lipids , Nanoparticles , การนำส่งยา , ลิปิด , อนุภาคนาโน
หน่วยงาน : จุฬาลงกรณ์มหาวิทยาลัย
ผู้ร่วมงาน : Garnpimol C. Ritthidej , Vimolmas Lipipun , Chulalongkorn University. Faculty of Pharmaceutical Sciences
ปีพิมพ์ : 2554
อ้างอิง : http://cuir.car.chula.ac.th/handle/123456789/49759
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Thesis (M.Sc.)--Chulalongkorn University, 2011

The purpose of this study was to prepare asiatic acid solid lipid nanoparticles (AA-SLN) for nasal delivery to brain. The SLN were preliminary prepared by hot high-pressure homogenization method composed of six solid lipids referred to trimyristin (TM), tistearin (TS), glyceryl behenate (GB), hydrogenated palm oil (SOF), palmitic acid (PA) and stearic acid (SA). Tween80 (TW80) was used as surfactant and poloxamer 188 (PL188), span80 (SP80), span85(SP85) and solutol HS15 (SL) were used as co-surfactants. After storage at accelerated condition, 25 formulations were maintained stabilized products. Palmitic acid and stearic acid could not produced stabilized formulations at all. Then asiatic acid was loaded SLN (AA-SLN) were prepared to determine physicochemical properties and stability at 4๐ C, room temperature and 45๐ C for 6 months. The particle size range from 34.53 + 0.81 to 186.50 + 2.23 nm. Larger sizes were obtained after coating with by chitosan. The zeta potential showed slightly negative charged and tended to slightly positive charged after coating with chitosan. The pH values were in the range of 4.5-6.5 which are suitable for used in nasal delivery route, they were slightly decreased when coating with chitosan. The entrapment efficiency of AA-SLN demonstrated high % entrapment efficiency of more than 95% w/w. Viscosity were in the range of 0.90 + 0.07 to 1.39 + 0.07 cps. The formulations showed newtonian flow characteristic that consistently exists between viscosity and shear rate as same as in CT/AA-SLN. The results of DSC and X-ray diffractions were corresponding similar that after AA was incorporated into SLN, the drug was not in crystalline state but in amorphous state. CT/AA-SLN displayed to enhance mucoadhesion property when compared with AA-SLN correlating to the concentration of added chitosan. The TEER values were not different after permeation experiments in all fomulations compared with initial. The % cell viability was more than 90% which correlated in cytotoxicity by MTT assay. TM TW80:SP85 5:5 was shown higher cellular uptake and permeation into human nasal epithelial carcinoma cell line than SOF TW80:SP85 5:5. CT/ AA-SLN that showed higher cellular uptake but lower permeation than AA-SLN which was agreement with in vitro permeation study thruogh 0.2 µm cellulose acetate membrane.

บรรณานุกรม :
Pilaiporn Khunathum . (2554). Development of asiatic acid solid lipid nanoparticles for nasal delivery.
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Pilaiporn Khunathum . 2554. "Development of asiatic acid solid lipid nanoparticles for nasal delivery".
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย.
Pilaiporn Khunathum . "Development of asiatic acid solid lipid nanoparticles for nasal delivery."
    กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย, 2554. Print.
Pilaiporn Khunathum . Development of asiatic acid solid lipid nanoparticles for nasal delivery. กรุงเทพมหานคร : จุฬาลงกรณ์มหาวิทยาลัย; 2554.