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Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle

หน่วยงาน มหาวิทยาลัยเชียงใหม่

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ชื่อเรื่อง : Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle
นักวิจัย : Duangmano,S. , Dakeng,S. , Jiratchariyakul,W. , Suksamrarn,A. , Smith,D.R. , Patmasiriwat,P.
คำค้น : Catalysis , Computer Science Applications , Spectroscopy , Organic Chemistry , Molecular Biology , Physical and Theoretical Chemistry , Inorganic Chemistry
หน่วยงาน : มหาวิทยาลัยเชียงใหม่
ผู้ร่วมงาน : -
ปีพิมพ์ : 2553
อ้างอิง : 14220067 , 2-s2.0-78650985401 , 10.3390/ijms11125323 , http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=78650985401&origin=inward , http://cmuir.cmu.ac.th/handle/6653943832/38090
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : -
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Naturally occurring cucurbitacins have been shown to have anticancer, antimicrobial and anti-inflammatory activities. In this study, we determined the effects of cucurbitacin B extracted from the Thai herb Trichosanthes cucumerina L. on telomerase regulation in three human breast cancer cell lines (T47D, SKBR-3, and MCF-7) and a mammary epithelium cell line (HBL-100). Cell viability after treatment with cucurbitacin B, which is an active ingredient of this herb, was assessed. Telomeric Repeat Amplification Protocol (TRAP) assays and RT-PCR (qualitative and realtime) were performed to investigate activity of telomerase as well as expression of human telomerase reverse transcriptase (hTERT) and c-Myc. The c-Myc protein level was also determined in SKBR-3 and HBL-100 cells. Our results show that the cucurbitacin B inhibits growth and telomerase activity in the three breast cancer cell lines and exerts an obvious inhibitory effect in the estrogen receptor (ER)-negative breast cancer SKBR-3 cells. The expression of hTERT and c-Myc were also inhibited by cucurbitacin B, In addition, a clear reduction of c-Myc protein was observed after treatment in SKBR-3 cells even with a concentration of cucurbitacin B that was ten-times lower compared to the concentration used for HBL-100. Our findings imply that cucurbitacin B exerts an anticancer effect by inhibiting telomerase via down regulating both the hTERT and c-Myc expression in breast cancer cells. © 2010 by the authors.

บรรณานุกรม :
Duangmano,S. , Dakeng,S. , Jiratchariyakul,W. , Suksamrarn,A. , Smith,D.R. , Patmasiriwat,P. . (2553). Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle.
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Duangmano,S. , Dakeng,S. , Jiratchariyakul,W. , Suksamrarn,A. , Smith,D.R. , Patmasiriwat,P. . 2553. "Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle".
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ .
Duangmano,S. , Dakeng,S. , Jiratchariyakul,W. , Suksamrarn,A. , Smith,D.R. , Patmasiriwat,P. . "Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle."
    เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ , 2553. Print.
Duangmano,S. , Dakeng,S. , Jiratchariyakul,W. , Suksamrarn,A. , Smith,D.R. , Patmasiriwat,P. . Antiproliferative effects of cucurbitacin B in breast cancer cells: Down-regulation of the c-Myc/hTERT/telomerase pathway and obstruction of the cell cycle. เชียงใหม่ : มหาวิทยาลัยเชียงใหม่ ; 2553.