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Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status

หน่วยงาน Southeast Asian Fisheries Development Center, Philippines

รายละเอียด

ชื่อเรื่อง : Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status
นักวิจัย : Pedroso, Fiona L. , De Jesus-Ayson, Evelyn Grace T. , Cortado, Hanna H. , Hyodo, Susumu , Ayson, Felix G.
คำค้น : Hormones , Starvation , Nutrition , Endocrinology , Growth rate , Food availability , Marine fish , Condition factor , Growth hormone , Insulin-like growth factor I , Nutritional status , Gene expression , Dietary restrictions , Signal peptides , Liver , Polyadenylation , Amino acids , Base pairs , Pituitary , Polymerase chain reaction , Primers , Open reading frames , mRNA
หน่วยงาน : Southeast Asian Fisheries Development Center, Philippines
ผู้ร่วมงาน : -
ปีพิมพ์ : 2549
อ้างอิง : Pedroso, F. L., de Jesus-Ayson, E. G. T., Cortado, H. H., Hyodo, S., & Ayson, F. G. (2006). Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status. General and Comparative Endocrinology, 145(3), 237-246. , 0016-6480 , http://hdl.handle.net/10862/94 , 10.1016/j.ygcen.2005.09.001
ที่มา : -
ความเชี่ยวชาญ : -
ความสัมพันธ์ : General and Comparative Endocrinology , General and Comparative Endocrinology 145:237-246 , http://scsagr.scsfri.ac.cn/upimg/200853095541.pdf
ขอบเขตของเนื้อหา : -
บทคัดย่อ/คำอธิบาย :

Growth hormone (GH) and insulin-like growth factor-I (IGF-I) are key links to nutritional condition and growth regulation in teleost. To understand the endocrine mechanism of growth regulation in grouper, we cloned the cDNAs for grouper GH and IGF-I and examined their mRNA expression during different nutritional status. Grouper GH cDNA is 936 base pairs (bp) long excluding the poly-A tail. It contained untranslated regions of 85 and 231bp in the 5'- and 3'-ends, respectively. It has an open reading frame of 612bp coding for a signal peptide of 17 amino acids (aa) and a mature hormone of 187aa residues. Based on the aa sequence of the mature hormone, grouper GH shows higher sequence identity (>76%) to GHs of perciforms than to GHs of cyprinids and salmonids (53-69%). Grouper preproIGF-I cDNA consisted of 558bp, which codes for 186aa. This is composed of 44aa for the signal peptide, 68aa for the mature peptide comprising B, C, A, and D domains, and 74aa for the E domain. Mature grouper IGF-I shows very high sequence identity to IGF-I of teleost fishes (84-97%) compared to advanced groups of vertebrates such as chicken, pig, and human (=<80%). Using DNA primers specific for grouper GH and IGF-I, the changes in mRNA levels of pituitary GH and hepatic IGF-I in response to starvation and refeeding were examined by a semi-quantitative RT-PCR. Significant elevation of GH mRNA level was observed after 2 weeks of food deprivation, and increased further after 3 and 4 weeks of starvation. GH mRNA level in fed-controls did not change significantly during the same period. Hepatic IGF-I mRNA level decreased significantly starting after 1 week of starvation until the 4th week. There was no significant change in IGF-I mRNA levels in fed-controls. One week of refeeding can restore the GH and IGF-I mRNA back to its normal levels. Deprivation of food for 1-4 weeks also resulted in cessation of growth and decrease in condition factor.

บรรณานุกรม :
Pedroso, Fiona L. , De Jesus-Ayson, Evelyn Grace T. , Cortado, Hanna H. , Hyodo, Susumu , Ayson, Felix G. . (2549). Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status.
    : Southeast Asian Fisheries Development Center, Philippines.
Pedroso, Fiona L. , De Jesus-Ayson, Evelyn Grace T. , Cortado, Hanna H. , Hyodo, Susumu , Ayson, Felix G. . 2549. "Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status".
    : Southeast Asian Fisheries Development Center, Philippines.
Pedroso, Fiona L. , De Jesus-Ayson, Evelyn Grace T. , Cortado, Hanna H. , Hyodo, Susumu , Ayson, Felix G. . "Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status."
    : Southeast Asian Fisheries Development Center, Philippines, 2549. Print.
Pedroso, Fiona L. , De Jesus-Ayson, Evelyn Grace T. , Cortado, Hanna H. , Hyodo, Susumu , Ayson, Felix G. . Changes in mRNA expression of grouper (Epinephelus coioides) growth hormone and insulin-like growth factor I in response to nutritional status. : Southeast Asian Fisheries Development Center, Philippines; 2549.